Project Name Metagenomic analysis of viruses in reclaimed water 
Domain (1) viruses
Investigators (1)
Institution University of South Florida
Project Code CAM_PROJ_ReclaimedWaterVirues
Project Type metagenome
Publications (1)


Reclaimed water use is an important component of sustainable water resource management. However, there are concerns regarding pathogen transport through this alternative water supply. This study characterized the viral community found in reclaimed water and compared it with viruses in potable water. Reclaimed water contained 1000-fold more viruslike particles than potable water, having approximately 108 VLPs per millilitre. Metagenomic analyses revealed that most of the viruses in both reclaimed and potable water were novel. Bacteriophages dominated the DNA viral community in both reclaimed and potable water, but reclaimed water had a distinct phage community based on phage family distributions and host representation within each family. Eukaryotic viruses similar to plant pathogens and invertebrate picornaviruses dominated RNA metagenomic libraries. Established human pathogens were not detected in reclaimed water viral metagenomes, which contained a wealth of novel single-stranded DNA and RNA viruses related to plant, animal and insect viruses. Therefore, reclaimed water may play a role in the dissemination of highly stable viruses. Information regarding viruses present in reclaimed water but not in potable water can be used to identify new bioindicators of water quality. Future studies will need to investigate the infectivity and host range of these viruses to evaluate the impacts of reclaimed water use on human and ecosystem health.


No assemblies.


Sample Name PI Desc Cart
RECLAIMEDWATERVIRUES_SMPL_SRX003360_EFFLUENTDNA NA Effluent Reclaimed Water DNA
RECLAIMEDWATERVIRUES_SMPL_SRX003361_EFFLUENTRNA NA Effluent Reclaimed Water cDNA
RECLAIMEDWATERVIRUES_SMPL_SRX003362_NURSERYDNA NA Nursery Reclaimed Water DNA
RECLAIMEDWATERVIRUES_SMPL_SRX003363_NURSERYRNA NA Nursery Reclaimed Water cDNA
RECLAIMEDWATERVIRUES_SMPL_SRX003364_PARKDNA NA Park Reclaimed Water DNA
RECLAIMEDWATERVIRUES_SMPL_SRX003365_POTALDNA NA Potal Water DNA